Persistent Neutrophil Infiltration and Unique Ocular Surface Microbiome Typify Dupilumab-Associated Conjunctivitis in Patients with Atopic Dermatitis.

ABSTRACT:

Objective

To elucidate the pathogenesis of dupilumab (Dupixent®)-associated ocular surface disease (DAOSD).

Design

Prospective single-center cohort study.

Subjects

Twenty patients with moderate-to-severe atopic dermatitis (AD) who received dupilumab and 10 age- and sex-matched healthy controls were enrolled in the study.

Methods

The study cohort underwent a thorough slit-lamp and entire-body dermatologic examination. Conjunctival swabs and smears were collected at baseline, 4 and 16 weeks after treatment initiation, and during the conjunctivitis episode. To analyse the ocular surface microbiome, 16S ribosomal RNA sequencing was performed, smears were hematoxylin and eosin stained, and serum cytokines were measured by using a multiplex immunobead assay.

Main

SUBMITTER: Patra V 

PROVIDER: S-EPMC10585475 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature