Cell Count and Cell Density Decrease as Age Increases in Cadaveric Pediatric Medial Menisci.

ABSTRACT:

Purpose

To examine the histologic changes in terms of cellularity, cell density, and nuclear shape in medial meniscal cellularity during skeletal development using pediatric cadaver specimens.

Methods

Medial menisci from 26 pediatric cadavers, 11 female and 15 male (total 36 menisci), were obtained from tissue bank. Mean age of female donors was 34 months (1-108 months) and of male donors was 52 months (1-132 months). Menisci were processed and embedded in paraffin blocks. Each tissue block containing 6 representative areas of meniscus (anterior root, anterior horn, body [n = 2], posterior horn, and posterior root) was sectioned at 4 microns and stained with hematoxylin and eosin for evaluation of chondrocyte nuclei. Each of the 6 representative areas was imaged at 10×; one

SUBMITTER: Albersheim M 

PROVIDER: S-EPMC10585640 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature