BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8⁺ T cell infiltration in colorectal cancer.

ABSTRACT:

Background

BRAF non-V600 mutation occupies a relatively small but critical subset in colorectal cancer (CRC). However, little is known about the biological functions and impacts of BRAF class III mutation in CRC. Here, we aim to explore how D594A mutation impacts on biological behaviors and immune related signatures in murine CRC cells.

Methods

BRAF V600E (class I), G469V (class II) and D594A (class III) mutant cell lines were established based on MC38 cells. The biological behaviors of cells were evaluated in respect of cell growth, cell proliferation, cell apoptosis, cell migration and invasion by the methods of colony-forming assay, CCK-8 assay, Annexin V/PI staining and transwell assay. The concentrations of soluble cytokines were detected by ELISA. The membrane expressi

SUBMITTER: Li W 

PROVIDER: S-EPMC10585750 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature