Cryopreservation and validation of differentiated PDGFRα-positive cells for long term usage in experimentation.

ABSTRACT:

Objective

Differentiation of immortalized Mesenchymal Stromal Cells (iMSCs) into PDGFRα-positive cells under controlled growth conditions has several vital implications in functional studies concerned with the pathogenesis of Diabetic Gastroparesis (DGP). A study published previously by our research group demonstrated the importance of these cells as a novel, in-vitro model for investigating the functional role of neuronal nitric oxide synthase. The currently available methods require fresh differentiation of PDGFRα-positive cells for each round of experimentation. This leads to longer delays, higher usage of reagents, and inconsistency in reproducibility of experiments frequently. We thus aimed to establish through validation that cryopreserving and maintaining the iMSC-derived PD

SUBMITTER: Srivastava A 

PROVIDER: S-EPMC10585902 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature