Type 2 cytokines sensitize human sensory neurons to itch-associated stimuli.

ABSTRACT:

Introduction

Chronic itch is a central symptom of atopic dermatitis. Cutaneous afferent neurons express receptors interleukins (IL)-4, IL-13, and IL-33, which are type 2 cytokines that are elevated in atopic dermatitis. These neuronal cytokine receptors were found to be required in several murine models of itch. Prior exposure of neurons to either IL-4 or IL-33 increased their response to subsequent chemical pruritogens in mice but has not been previously examined in humans. The objective of the present study was to determine if type 2 cytokine stimulation sensitizes sensory neurons to future itch stimuli in a fully human ex vivo system.

Methods

We measured calcium flux from human dorsal root ganglia cultures from cadaveric donors in response to pruritogens following

SUBMITTER: Mack MR 

PROVIDER: S-EPMC10586051 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature