Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation.

ABSTRACT: The intersection of protein and lipid biology is of growing importance for understanding how cells address structural challenges during adhesion and migration. While protein complexes engaged with the cytoskeleton play a vital role, support from the phospholipid membrane is crucial for directing localization and assembly of key protein complexes. During angiogenesis, dramatic cellular remodeling is necessary for endothelial cells to shift from a stable monolayer to invasive structures. However, the molecular dynamics between lipids and proteins during endothelial invasion are not defined. Here, we utilized cell culture, immunofluorescence, and lipidomic analyses to identify a novel role for the membrane binding protein Annexin A2 (ANXA2) in modulating the composition of specific membrane l

SUBMITTER: Sveeggen TM 

PROVIDER: S-EPMC10586062 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature