Identification and validation of key genes associated with pathogenesis and prognosis of gastric cancer.

ABSTRACT:

Background

Gastric cancer (GC) is the fourth leading cause of cancer-related death worldwide. However, the precise mechanisms and specific biomarkers of GC have not been fully elucidated. We therefore sought to identify and validate the genes associated with GC.

Methods

RNA sequencing was performed on gastric tissue specimens from 10 cases each of non-atrophic gastritis (NAG), intestinal metaplasia (IM), and GC. Validation of gene expression was conducted through immunohistochemistry (IHC) staining. The Kaplan-Meier Plotter database was utilized to screen genes associated with prognosis, while protein-protein interaction analysis was conducted to identify hub genes.

Results

In GC-IM, the differentially expressed genes (DEGs) were predominantly enriched in pathways rel

SUBMITTER: Li A 

PROVIDER: S-EPMC10586292 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature