Cytosolic Protein Delivery Using Modular Biotin-Streptavidin Assembly of Nanocomposites.

ABSTRACT: Current strategies for the delivery of proteins into cells face general challenges of endosomal entrapment and concomitant degradation of protein cargo. Efficient delivery directly to the cytosol overcomes this obstacle: we report here the use of biotin-streptavidin tethering to provide a modular approach to the generation of nanovectors capable of a cytosolic delivery of biotinylated proteins. This strategy uses streptavidin to organize biotinylated protein and biotinylated oligo(glutamate) peptide into modular complexes that are then electrostatically self-assembled with a cationic guanidinium-functionalized polymer. The resulting polymer-protein nanocomposites demonstrate efficient cytosolic delivery of six biotinylated protein cargos of varying size, charge, and quaternary structure. R

SUBMITTER: Luther DC 

PROVIDER: S-EPMC10586328 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature