Utility of nasal swabs for assessing mucosal immune responses towards SARS-CoV-2.

ABSTRACT: SARS-CoV-2 has caused millions of infections worldwide since its emergence in 2019. Understanding how infection and vaccination induce mucosal immune responses and how they fluctuate over time is important, especially since they are key in preventing infection and reducing disease severity. We established a novel methodology for assessing SARS-CoV-2 cytokine and antibody responses at the nasal epithelium by using nasopharyngeal swabs collected longitudinally before and after either SARS-CoV-2 infection or vaccination. We then compared responses between mucosal and systemic compartments. We demonstrate that cytokine and antibody profiles differ between compartments. Nasal cytokines show a wound healing phenotype while plasma cytokines are consistent with pro-inflammatory pathways. We found

SUBMITTER: Roubidoux EK 

PROVIDER: S-EPMC10587113 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature