Structural characterization, biofunctionality, and environmental factors impacting rheological properties of exopolysaccharide produced by probiotic Lactococcus lactis C15.

ABSTRACT: Exopolysaccharides (EPSs) possess distinctive rheological and physicochemical properties and innovative functionality. This study aimed to investigate the physicochemical, bioactive, and rheological properties of an EPS secreted by Lactococcus lactis subsp. lactis C15. EPS-C15 was found to have an average molecular weight of 8.8 × 10⁵ Da and was identified as a hetero-EPS composed of arabinose, xylose, mannose, and glucose with a molar ratio of 2.0:2.7:1.0:21.3, respectively. The particle size and zeta potential represented 311.2 nm and - 12.44 mV, respectively. FITR exhibited that EPS-C15 possessed a typical polysaccharide structure. NMR displayed that EPS-C15 structure is → 3)α-d-Glc^vi (1 → 3)α-d-Xyl^v (1 → 6)α-d-Glc^iv(1 → 4)α-d-Glc(1 → 3)β-d-Ma

SUBMITTER: Bamigbade G 

PROVIDER: S-EPMC10587178 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature