Neutralizing antibodies from prior exposure to dengue virus negatively correlate with viremia on re-infection.

ABSTRACT:

Background

India is hyperendemic to dengue and over 50% of adults are seropositive. There is limited information on the association between neutralizing antibody profiles from prior exposure and viral RNA levels during subsequent infection.

Methods

Samples collected from patients with febrile illness was used to assess seropositivity by indirect ELISA. Dengue virus (DENV) RNA copy numbers were estimated by quantitative RT-PCR and serotype of the infecting DENV was determined by nested PCR. Focus reduction neutralizing antibody titer (FRNT) assay was established using Indian isolates to measure the levels of neutralizing antibodies and also to assess the cross-reactivity to related flaviviruses namely Zika virus (ZIKV), Japanese encephalitis virus (JEV) and West Nile virus (W

SUBMITTER: Anantharaj A 

PROVIDER: S-EPMC10587183 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature