Identification of a gene set that maintains tumorigenicity of the hepatocellular carcinoma cell line Li-7.

ABSTRACT: The identification and development of therapeutic targets in cancer stem cells that lead to tumor development, recurrence, metastasis, and drug resistance is an important goal in cancer research. The hepatocellular carcinoma cell line Li-7 contains functionally different types of cells. Cells with tumor-forming activity are enriched in cancer stem cell-like CD13⁺CD166^- cells and this cell population gradually decreases during culture in conventional culture medium (RPMI1640 containing 10% fetal bovine serum). When Li-7 cells are cultured in mTeSR1, a medium developed for human pluripotent stem cells, CD13⁺CD166^- cells, and their tumorigenicity is maintained. Here, we sought to identify the mechanisms of tumorigenicity in this sub-population. We c

SUBMITTER: Seyama Y 

PROVIDER: S-EPMC10587214 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature