Passive diffusion accounts for the majority of intracellular nanovesicle transport.

ABSTRACT: During membrane trafficking, a vesicle formed at the donor compartment must travel to the acceptor membrane before fusing. For large carriers, it is established that this transport is motor-driven; however, the mode by which small vesicles, which outnumber larger carriers, are transported is poorly characterized. Here, we show that intracellular nanovesicles (INVs), a substantial class of small vesicles, are highly mobile within cells and that this mobility depends almost entirely on passive diffusion (0.1-0.3 μm² s^-1). Using single particle tracking, we describe how other small trafficking vesicles have a similar diffusive mode of transport that contrasts with the motor-dependent movement of larger endolysosomal carriers. We also demonstrate that a subset of INVs is

SUBMITTER: Sittewelle M 

PROVIDER: S-EPMC10587482 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature