DNA metabarcoding assessment of Neotropical ichthyoplankton communities is marker-dependent.

ABSTRACT: The study of ichthyoplankton is paramount to understanding fish assemblages' reproductive dynamics. DNA metabarcoding has been applied as a rapid, cost-effective, and accurate taxonomy tool, allowing the identification of multiple individuals simultaneously. However, there remain significant challenges when using DNA metabarcoding, such as molecular marker choice according to the taxonomic resolution and length of the fragment to be sequenced, primer bias, incomplete reference databases, and qualitative inference incongruences. Here, 30 ichthyoplankton pools collected from a Neotropical river were identified at a molecular level using DNA metabarcoding to compare the resolution, sensibility, specificity, and relative read abundance (RRA) recovery of three molecular markers: the standard CO

SUBMITTER: Teixeira DF 

PROVIDER: S-EPMC10587807 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature