A Polymer-Based Multichannel Sensor for Rapid Cell-Based Screening of Antibiotic Mechanisms and Resistance Development.

ABSTRACT: Antibiotic resistance presents a critical threat to public health, necessitating the rapid development of novel antibiotics and an appropriate choice of therapeutics to combat refractory bacterial infections. Here, we report a high-throughput polymer-based sensor platform that rapidly (30 min) profiles mechanisms of antibiotic activity. The sensor array features three fluorophore-conjugated polymers that can detect subtle antibiotic-induced phenotypic changes on bacterial surfaces, generating distinct mechanism-based fluorescence patterns. Notably, discrimination of different generations of antibiotic resistance was achieved with high efficiency. This sensor platform combines trainability, simplicity, and rapid screening into a readily translatable platform.

SUBMITTER: Basu Roy S 

PROVIDER: S-EPMC10587897 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature