Patient-derived organoid culture in epithelial ovarian cancers-Techniques, applications, and future perspectives.

ABSTRACT: Epithelial ovarian cancer (EOC) is a heterogeneous disease composed of different cell types with different molecular aberrations. Traditional cell lines and mice models cannot recapitulate the human tumor biology and tumor microenvironment (TME). Patient-derived organoids (PDOs) are freshly derived from patients' tissues and are then cultured with extracellular matrix and conditioned medium. The high concordance of epigenetic, genomic, and proteomic landscapes between the parental tumors and PDOs suggests that PDOs can provide more reliable results in studying cancer biology, allowing high throughput drug screening, and identifying their associated signaling pathways and resistance mechanisms. However, despite having a heterogeneity of cells in PDOs, some cells in TME will be lost during t

SUBMITTER: Chan WS 

PROVIDER: S-EPMC10587945 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature