Genetic screening for anticancer genes highlights FBLN5 as a synthetic lethal partner of MYC.

ABSTRACT:

Background

When ectopically overexpressed, anticancer genes, such as TRAIL, PAR4 and ORCTL3, specifically destroy tumour cells without harming untransformed cells. Anticancer genes can not only serve as powerful tumour specific therapy tools but studying their mode of action can reveal mechanisms underlying the neoplastic transformation, sustenance and spread.

Methods

Anticancer gene discovery is normally accidental. Here we describe a systematic, gain of function, forward genetic screen in mammalian cells to isolate novel anticancer genes of human origin. Continuing with over 30,000 transcripts from our previous study, 377 cell death inducing genes were subjected to screening. FBLN5 was chosen, as a proof of principle, for mechanistic gene expression profiling, comparison p

SUBMITTER: Masood M 

PROVIDER: S-EPMC10588048 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature