NR4A1 deletion promotes pro-angiogenic polarization of macrophages derived from classical monocytes in a mouse model of neovascular age-related macular degeneration.

ABSTRACT:

Background

Neovascular age-related macular degeneration causes vision loss from destructive angiogenesis, termed choroidal neovascularization (CNV). Cx3cr1^-/- mice display alterations in non-classical monocytes and microglia with increased CNV size, suggesting that non-classical monocytes may inhibit CNV formation. NR4A1 is a transcription factor that is necessary for maturation of non-classical monocytes from classical monocytes. While Nr4a1^-/- mice are deficient in non-classical monocytes, results are confounded by macrophage hyper-activation. Nr4a1^se2/se2 mice lack a transcriptional activator, resulting in non-classical monocyte loss without macrophage hyper-activation.

Main body

We subjected Nr4a1^-/- and Nr4a1^se2/se2 mice to the laser-induced CNV model and performed multi-parameter flow cytometry. We found that both models lack non-classical monocytes, but only Nr4a1^-/- mice displayed increased CNV area. Additionally, CD11c⁺ macrophages were increased in Nr4a1^-/- mice. Single-cell transcriptomic analysis uncovered that CD11c⁺ macrophages were enriched from Nr4a1^-/- mice and expressed a pro-angiogenic transcriptomic profile that was disparate from prior reports of macrophage hyper-activation.

Conclusions

These results suggest that non-classical monocytes are dispensable during CNV, and NR4A1 deficiency results in increased recruitment of pro-angiogenic macrophages.

SUBMITTER: Droho S 

PROVIDER: S-EPMC10588116 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature