Impairing Gasdermin D-mediated pyroptosis is protective against retinal degeneration.

ABSTRACT:

Background

Inflammasome activation and the subsequent release of pro-inflammatory cytokines including Interleukin 1β (IL-1β) have been widely reported to contribute to the progression of retinal degenerations, including age-related macular degeneration (AMD), the leading cause of blindness in the Western World. The role of Gasdermin D (GSDMD), a key executioner of pyroptosis following inflammasome activation, however, is less well-established. In this study we aimed to characterise the role of GSDMD in the healthy and degenerating retina, and uncover its role as a conduit for IL-1β release, including via extracellular vesicle (EV)-mediated release.

Methods

GSDMD mutant and knockout mice, in vitro models of inflammation and a well-established in vivo model of retinal degenera

SUBMITTER: Sekar R 

PROVIDER: S-EPMC10588253 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature