Can Single Cell Respiration be Measured by Scanning Electrochemical Microscopy (SECM)?

ABSTRACT: Ultramicroelectrode (UME), or, equivalently, microelectrode, probes are increasingly used for single-cell measurements of cellular properties and processes, including physiological activity, such as metabolic fluxes and respiration rates. Major challenges for the sensitivity of such measurements include: (i) the relative magnitude of cellular and UME fluxes (manifested in the current); and (ii) issues around the stability of the UME response over time. To explore the extent to which these factors impact the precision of electrochemical cellular measurements, we undertake a systematic analysis of measurement conditions and experimental parameters for determining single cell respiration rates via the oxygen consumption rate (OCR) in single HeLa cells. Using scanning electrochemical microscop

SUBMITTER: Cremin K 

PROVIDER: S-EPMC10588932 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature