SYCP1 head-to-head assembly is required for chromosome synapsis in mouse meiosis.

ABSTRACT: In almost all sexually reproducing organisms, meiotic recombination and cell division require the synapsis of homologous chromosomes by a large proteinaceous structure, the synaptonemal complex (SC). While the SC's overall structure is highly conserved across eukaryotes, its constituent proteins diverge between phyla. Transverse filament protein, SYCP1, spans the width of the SC and undergoes amino-terminal head-to-head self-assembly in vitro through a motif that is unusually highly conserved across kingdoms of life. Here, we report creation of mouse mutants, Sycp1^L102E and Sycp1^L106E, that target SYCP1's head-to-head interface. L106E resulted in a complete loss of synapsis, while L102E had no apparent effect on synapsis, in agreement with their differen

SUBMITTER: Billmyre KK 

PROVIDER: S-EPMC10588951 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature