Structural foundation for the role of enterococcal PrgB in conjugation, biofilm formation, and virulence.

ABSTRACT: Type 4 Secretion Systems are a main driver for the spread of antibiotic resistance genes and virulence factors in bacteria. In Gram-positives, these secretion systems often rely on surface adhesins to enhance cellular aggregation and mating-pair formation. One of the best studied adhesins is PrgB from the conjugative plasmid pCF10 of Enterococcus faecalis, which has been shown to play major roles in conjugation, biofilm formation, and importantly also in bacterial virulence. Since prgB orthologs exist on a large number of conjugative plasmids in various different species, this makes PrgB a model protein for this widespread virulence factor. After characterizing the polymer adhesin domain of PrgB previously, we here report the structure for almost the entire remainder of PrgB,

SUBMITTER: Sun WS 

PROVIDER: S-EPMC10588982 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature