Exploring virus presence in field-collected potato leaf samples using RNA sequencing.

ABSTRACT:

Background

The quick and accurate identification of viruses is essential for plant disease management. Next-generation sequencing (NGS) technology may allow the discovery, detection, and identification of plant pathogens. This study adopted RNA-sequencing (RNA-Seq) technology to explore the viruses in three potato plants (S3, S4, and S6) growing under field conditions.

Results

Potato-known infecting viruses, such as alfalfa mosaic virus (AMV), potato leafroll virus (PLRV), and potato virus Y (PVY), were identified using bioinformatics programs and validated using RT-PCR. The presence of these potato viruses was also confirmed by visual inspection of host symptoms. In addition, the nearly complete genome of PLRV and the complete or partial genome sequence of multipartite viru

SUBMITTER: Elwan EA 

PROVIDER: S-EPMC10589165 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature