Novel findings about the mode of action of the antifungal protein PeAfpA against Saccharomyces cerevisiae.

ABSTRACT: Antifungal proteins (AFPs) from filamentous fungi offer the potential to control fungal infections that threaten human health and food safety. AFPs exhibit broad antifungal spectra against harmful fungi, but limited knowledge of their killing mechanism hinders their potential applicability. PeAfpA from Penicillium expansum shows strong antifungal potency against plant and human fungal pathogens and stands above other AFPs for being active against the yeast Saccharomyces cerevisiae. We took advantage of this and used a model laboratory strain of S. cerevisiae to gain insight into the mode of action of PeAfpA by combining (i) transcriptional profiling, (ii) PeAfpA sensitivity analyses of deletion mutants available in the S. cerevisiae genomic deletion collection and (iii) cell biology studie

SUBMITTER: Giner-Llorca M 

PROVIDER: S-EPMC10589166 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature