Loop-mediated isothermal amplification assay for screening congenital cytomegalovirus infection in newborns.

ABSTRACT: Congenital cytomegalovirus (CMV) infection is a common cause of sensorineural hearing loss and neurodevelopmental impairment in newborns. However, congenital CMV infection cannot be diagnosed using samples collected more than 3 weeks after birth because testing after this time cannot distinguish between congenital infection and postnatal infection. Herein, we developed a robust loop-mediated isothermal amplification (LAMP) assay for the large-scale screening of newborns for congenital CMV infection. In contrast to conventional quantitative polymerase chain reaction (qPCR), which detects CMV within a dynamic range of 1.0 × 10⁶ to 1.0 × 10² copies/μL, our quantitative LAMP assay (qLAMP) detects CMV within a dynamic range of 1.1 × 10⁸ to 1.1 × 10³ c

SUBMITTER: Park H 

PROVIDER: S-EPMC10589182 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature