Pairwise biosynthesis of ion channels stabilizes excitability and mitigates arrhythmias.

ABSTRACT: Coordinated expression of ion channels is crucial for cardiac rhythms, neural signaling, and cell cycle progression. Perturbation of this balance results in many disorders including cardiac arrhythmias. Prior work revealed association of mRNAs encoding cardiac Na_V1.5 (SCN5A) and hERG1 (KCNH2), but the functional significance of this association was not established. Here, we provide a more comprehensive picture of KCNH2, SCN5A, CACNA1C, and KCNQ1 transcripts collectively copurifying with nascent hERG1, Na_V1.5, Ca_V1.2, or KCNQ1 channel proteins. Single-molecule fluorescence in situ hybridization (smFISH) combined with immunofluorescence reveals that the channel proteins are synthesized predominantly as heterotypic pai

SUBMITTER: Jameson MB 

PROVIDER: S-EPMC10589643 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature