Progenitor division and cell autonomous neurosecretion are required for rod photoreceptor sublaminar positioning.

ABSTRACT: Migration is essential for the laminar stratification and connectivity of neurons in the central nervous system. In the retina, photoreceptors (PRs) migrate to positions according to birthdate, with early-born cells localizing to the basal-most side of the outer nuclear layer. It was proposed that apical progenitor mitoses physically drive these basal translocations non-cell autonomously, but direct evidence is lacking, and whether other mechanisms participate is unknown. Here, combining loss- or gain-of-function assays to manipulate cell cycle regulators (Sonic hedgehog, Cdkn1a/p21) with an in vivo lentiviral labelling strategy, we demonstrate that progenitor division is one of two forces driving basal translocation of rod soma. Indeed, replacing Shh activity rescues abnormal rod transloc

SUBMITTER: Gurdita A 

PROVIDER: S-EPMC10589646 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature