Knockout of STE20-type kinase TAOK3 does not attenuate diet-induced NAFLD development in mice.

ABSTRACT:

Objective

Non-alcoholic fatty liver disease (NAFLD), the primary hepatic consequence of obesity, is affecting about 25% of the global adult population. The aim of this study was to examine the in vivo role of STE20-type protein kinase TAOK3, which has been previously reported to regulate hepatocellular lipotoxicity in vitro, in the development of NAFLD and systemic insulin resistance in the context of obesity.

Methods

Taok3 knockout mice and wild-type littermates were challenged with a high-fat diet. Various in vivo tests were performed to characterize the whole-body metabolism. NAFLD progression in the liver, and lipotoxic damage in adipose tissue, kidney, and skeletal muscle were compared between the genotypes by histological assessment, immunofluorescence microscopy, prot

SUBMITTER: Xia Y 

PROVIDER: S-EPMC10589923 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature