Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflows.

ABSTRACT:

Background

Long read sequencing allows the analysis of full-length transcripts in plants without the challenges of reliable transcriptome assembly. Long read sequencing of transcripts from plant genomes has often utilized sized transcript libraries. However, the value of including libraries of differing sizes has not been established.

Methods

A comprehensive transcriptome of the leaves of Jojoba (Simmondsia chinensis) was generated from two different PacBio library preparations: standard workflow (SW) and long workflow (LW).

Results

The importance of using both transcript groups in the analysis was demonstrated by the high proportion of unique sequences (74.6%) that were not shared between the groups. A total of 37.8% longer transcripts were only detected in the long

SUBMITTER: Al-Dossary O 

PROVIDER: S-EPMC10589961 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature