Functional integration of natural killer cells in a microfluidically perfused liver on-a-chip model.

ABSTRACT:

Objective

The liver acts as an innate immunity-dominant organ and natural killer (NK) cells, are the main lymphocyte population in the human liver. NK cells are in close interaction with other immune cells, acting as the first line of defense against pathogens, infections, and injury. A previously developed, three-dimensional, perfused liver-on-a-chip comprised of human cells was used to integrate NK cells, representing pivotal immune cells during liver injury and regeneration. The objective of this study was to integrate functional NK cells in an in vitro model of the human liver and assess utilization of the model for NK cell-dependent studies of liver inflammation.

Results

NK cells from human blood and liver specimen were isolated by Percoll separation with subsequent mag

SUBMITTER: Fahrner R 

PROVIDER: S-EPMC10590007 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature