IGF2BP2-meidated m⁶A modification of CSF2 reprograms MSC to promote gastric cancer progression.

ABSTRACT: The interaction between tumor cells and stromal cells within the tumor microenvironment plays a critical role in cancer progression. Mesenchymal stem cells (MSCs) are important tumor stromal cells that exhibit pro-oncogenic activities when reprogrammed by the tumor. However, the precise mechanisms underlying MSC reprogramming in gastric cancer remain not well understood. QRT-PCR, western blot, and immunohistochemistry were used to examine gene and protein expression levels. In vitro and in vivo experiments were conducted to assess the biological functions of gastric cancer cells. RNA-sequencing, RNA immunoprecipitation (RIP), and meRIP assays were performed to investigate underlying molecular mechanisms. We found a significant increase in the expression and N6-methyladenosine (m⁶

SUBMITTER: Ji R 

PROVIDER: S-EPMC10590395 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature