3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription.

ABSTRACT: In mammals, insulators contribute to the regulation of loop extrusion to organize chromatin into topologically associating domains. In Drosophila the role of insulators in 3D genome organization is, however, under current debate. Here, we addressed this question by combining bioinformatics analysis and multiplexed chromatin imaging. We describe a class of Drosophila insulators enriched at regions forming preferential chromatin interactions genome-wide. Notably, most of these 3D interactions do not involve TAD borders. Multiplexed imaging shows that these interactions occur infrequently, and only rarely involve multiple genomic regions coalescing together in space in single cells. Finally, we show that non-border preferential 3D interactions enriched in this class of insulators are present

SUBMITTER: Messina O 

PROVIDER: S-EPMC10590426 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature