Mass production of lumenogenic human embryoid bodies and functional cardiospheres using in-air-generated microcapsules.

ABSTRACT: Organoids are engineered 3D miniature tissues that are defined by their organ-like structures, which drive a fundamental understanding of human development. However, current organoid generation methods are associated with low production throughputs and poor control over size and function including due to organoid merging, which limits their clinical and industrial translation. Here, we present a microfluidic platform for the mass production of lumenogenic embryoid bodies and functional cardiospheres. Specifically, we apply triple-jet in-air microfluidics for the ultra-high-throughput generation of hollow, thin-shelled, hydrogel microcapsules that can act as spheroid-forming bioreactors in a cytocompatible, oil-free, surfactant-free, and size-controlled manner. Uniquely, we show that microc

SUBMITTER: van Loo B 

PROVIDER: S-EPMC10590445 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature