An experimental design for obtaining DNA of a target species and its diet from a single non-invasive genetic protocol.

ABSTRACT: Next-generation sequencing technology has enabled accurate insights into the diet of wildlife species. The protocols for faecal sample collection and DNA extraction for diet analysis have differed from those focusing on target species, even in most studies combining questions on both aspects. We designed an experiment to evaluate two protocols using 11 parameters and select a single one that will generate both target species (Asiatic wild ass, Equus hemionus, in Israel) and diet DNA, as an effective strategy to minimise time, effort, and cost without hampering efficiency. In Protocol A, we swabbed the outer surface of faecal boluses and extracted DNA using a Stool Kit, while for Protocol B, we homogenised faecal matter from inside the bolus followed by extraction using a Powersoil K

SUBMITTER: Paul S 

PROVIDER: S-EPMC10590962 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature