Compressive sensing of functional connectivity maps from patterned optogenetic stimulation of neuronal ensembles.

ABSTRACT: Mapping functional connectivity between neurons is an essential step toward probing the neural computations mediating behavior. Accurately determining synaptic connectivity maps in populations of neurons is challenging in terms of yield, accuracy, and experimental time. Here, we developed a compressive sensing approach to reconstruct synaptic connectivity maps based on random two-photon cell-targeted optogenetic stimulation and membrane voltage readout of many putative postsynaptic neurons. Using a biophysical network model of interconnected populations of excitatory and inhibitory neurons, we characterized mapping recall and precision as a function of network observability, sparsity, number of neurons stimulated, off-target stimulation, synaptic reliability, propagation latency, and netwo

SUBMITTER: Navarro P 

PROVIDER: S-EPMC10591201 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature