Acteoside protects podocyte against apoptosis through regulating AKT/GSK-3β signaling pathway in db/db mice.

ABSTRACT:

Background

Podocyte apoptosis is one of the important pathological mechanisms of diabetic kidney disease (DKD). Acteoside (Act), a major active component of Rehmannia glutinosa leaves total glycoside, has a strong renoprotective action. Our study aims to demonstrate Act's renoprotective actions in db/db mice.

Methods

We adopted C57BLKS/J db/db mice as DKD animal models. After 8 weeks of Act administration, the 24-hour urine albumin, renal function index, and blood lipid levels were quantified using matching kits. Renal pathology was evaluated by HE and PAS staining. The podocyte damage and apoptosis-related signaling pathway were observed by using immunohistochemistry, western blot, and TUNEL staining.

Results

The albuminuria of db/db mice was reduced from 391 ug/24 h

SUBMITTER: Li X 

PROVIDER: S-EPMC10591407 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature