FLASH-RT does not affect chromosome translocations and junction structures beyond that of CONV-RT dose-rates.

ABSTRACT:

Background and purpose

The impact of radiotherapy (RT) at ultra high vs conventional dose rate (FLASH vs CONV) on the generation and repair of DNA double strand breaks (DSBs) is an important question that remains to be investigated. Here, we tested the hypothesis as to whether FLASH-RT generates decreased chromosomal translocations compared to CONV-RT.

Materials and methods

We used two FLASH validated electron beams and high-throughput rejoin and genome-wide translocation sequencing (HTGTS-JoinT-seq), employing S. aureus and S. pyogenes Cas9 "bait" DNA double strand breaks (DSBs) in HEK239T cells, to measure differences in bait-proximal repair and their genome-wide translocations to "prey" DSBs generated after various irradiation doses, dose rates and oxygen tensions (normox

SUBMITTER: Barghouth PG 

PROVIDER: S-EPMC10591966 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature