Global proteomics reveals insulin abundance as a marker of human islet homeostasis alterations.

ABSTRACT:

Aim

The variation in quality between the human islet samples represents a major problem for research, especially when used as control material. The assays assessing the quality of human islets used in research are non-standardized and limited, with many important parameters not being consistently assessed. High-throughput studies aimed at characterizing the diversity and segregation markers among apparently functionally healthy islet preps are thus a requirement. Here, we designed a pilot study to comprehensively identify the diversity of global proteome signatures and the deviation from normal homeostasis in randomly selected human-isolated islet samples.

Methods

By using Tandem Mass Tag 16-plex proteomics, we focused on the recurrently observed disparity in the detected in

SUBMITTER: Mathisen AF 

PROVIDER: S-EPMC10592125 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature