C1 inhibitor and prolylcarboxypeptidase modulate prekallikrein activation on endothelial cells.

ABSTRACT:

Background

We examined how prekallikrein (PK) activation on human microvascular endothelial cells (HMVECs) is regulated by the ambient concentration of C1 inhibitor (C1INH) and prolylcarboxypeptidase (PRCP).

Objective

We sought to examine the specificity of PK activation on HMVECs by PRCP and the role of C1INH to regulate it, high-molecular-weight kininogen (HK) cleavage, and bradykinin (BK) liberation.

Methods

Investigations were performed on cultured HMVECs. Immunofluorescence, enzymatic activity assays, immunoblots, small interfering RNA knockdowns, and cell transfections were used to perform these studies.

Results

Cultured HMVECs constitutively coexpressed PK, HK, C1INH, and PRCP. PK activation on HMVECs was modulated by the ambient C1INH concentration. In

SUBMITTER: Merkulova AA 

PROVIDER: S-EPMC10592223 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature