Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance in Arabidopsis thaliana.

ABSTRACT:

Background

Plant-pathogen interactions occur in the apoplast comprising the cell wall matrix and the fluid in the extracellular space outside the plasma membrane. However, little is known regarding the contribution of the apoplastic proteome to systemic acquired resistance (SAR).

Methods

Specifically, SAR was induced by inoculating plants with Pst DC3000 avrRps4. The apoplast washing fluid (AWF) was collected from the systemic leaves of the SAR-induced or mock-treated plants. A label free quantitative proteomic analysis was performed to identified the proteins related to SAR in AWF.

Results

A total of 117 proteins were designated as differentially accumulated proteins (DAPs), including numerous pathogenesis-related proteins, kinases, glycosyl hydrolases, and re

SUBMITTER: Jiang S 

PROVIDER: S-EPMC10592298 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature