Impact of enzymatic digestion on single cell suspension yield from peripheral human lung tissue.

ABSTRACT: An increasing number of translational investigations of lung biology rely on analyzing single cell suspensions obtained from human lungs. To obtain these single cell suspensions, human lungs from biopsies or research-consented organ donors must be subjected to mechanical and enzymatic digestion prior to analysis with either flow cytometry or single cell RNA sequencing. A variety of enzymes have been used to perform tissue digestion, each with potential limitations. To better understand the limitations of each enzymatic digestion protocol and to establish a framework for comparing studies across protocols, we performed five commonly published protocols in parallel from identical samples obtained from 6 human lungs. Following mechanical (gentleMACS™) and enzymatic digestion, we quantified ce

SUBMITTER: Bondonese A 

PROVIDER: S-EPMC10592386 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature