Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds.

ABSTRACT: Macrophages are essential in the initiation, maintenance, and transition of inflammatory processes such as foreign body response and wound healing. Mounting evidence suggests that physical factors also modulate macrophage activation. 2D in vitro systems demonstrate that constraining macrophages to small areas or channels modulates their phenotypes and changes their responses to known inflammatory agents such as lipopolysaccharide. However, how dimensionality and pore size affect macrophage phenotype is less explored. In this work, the change in macrophage M1/M2 polarization when confined in microporous annealed particle (MAP) scaffolds is studied. Particles sizes (40, 70, and 130 µm) are selected using outputs from software LOVAMAP that analyzes the characteristics of 3D pores in MAP gels.

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10592513 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature