TMEM65 regulates NCLX-dependent mitochondrial calcium efflux.

ABSTRACT: The balance between mitochondrial calcium (_mCa²⁺) uptake and efflux regulates ATP production, but if perturbed causes energy starvation or _mCa²⁺ overload and cell death. The mitochondrial sodium-calcium exchanger, NCLX, is a critical route of _mCa²⁺ efflux in excitable tissues, such as the heart and brain, and animal models support NCLX as a promising therapeutic target to limit pathogenic _mCa²⁺ overload. However, the mechanisms that regulate NCLX activity remain largely unknown. We used proximity biotinylation proteomic screening to identify the NCLX interactome and define novel regulators of NCLX function. Here, we discover the mitochondrial inner membrane protein, TMEM65, as an NCLX-proximal protein t

SUBMITTER: Garbincius JF 

PROVIDER: S-EPMC10592617 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature