Square beams for optimal tiling in TEM.

ABSTRACT: Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem on dose-sensitive samples. Here, we introduce a square electron beam that can be easily retrofitted in existing microscopes and demonstrate its application, showing it can tile nearly perfectly and deliver cryo-EM imaging with a resolution comparable to conventional setups.

SUBMITTER: Chua EY 

PROVIDER: S-EPMC10592621 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature