Double-strand break repair-associated intragenic deletions and tandem duplications suggest the architecture of the repair replication fork.

ABSTRACT: Double-strand break (DSB) repair is associated with a 1000-fold increase in mutations compared to normal replication of the same sequences. In budding yeast, repair of an HO endonuclease-induced DSB at the MATα locus can be repaired by using a homologous, heterochromatic HMR::Kl-URA3 donor harboring a transcriptionally silenced URA3 gene, resulting in a MAT::URA3 (Ura⁺) repair product where URA3 is expressed. Repair-associated ura3- mutations can be selected by resistance to 5-fluoroorotic acid (FOA). Using this system, we find that a major class of mutations are -1 deletions, almost always in homonucleotide runs, but there are few +1 insertions. In contrast, +1 and -1 insertions in homonucleotide runs are nearly equal among spontaneous

SUBMITTER: Dalin S 

PROVIDER: S-EPMC10592705 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature