Ontology highlight
ABSTRACT:
SUBMITTER: Sinha J
PROVIDER: S-EPMC10592807 | biostudies-literature | 2023 Oct
REPOSITORIES: biostudies-literature
Sinha Joydeb J Nickels Jan F JF Thurm Abby R AR Ludwig Connor H CH Archibald Bella N BN Hinks Michaela M MM Wan Jun J Fang Dong D Bintu Lacramioara L
bioRxiv : the preprint server for biology 20231014
Histone H3.3 is frequently mutated in cancers, with the lysine 36 to methionine mutation (K36M) being a hallmark of chondroblastomas. While it is known that H3.3K36M changes the cellular epigenetic landscape, it remains unclear how it affects the dynamics of gene expression. Here, we use a synthetic reporter to measure the effect of H3.3K36M on silencing and epigenetic memory after recruitment of KRAB: a member of the largest class of human repressors, commonly used in synthetic biology, and ass ...[more]