Inferring B cell phylogenies from paired heavy and light chain BCR sequences with Dowser.

ABSTRACT: Antibodies are vital to human immune responses and are composed of genetically variable heavy and light chains. These structures are initially expressed as B cell receptors (BCRs). BCR diversity is shaped through somatic hypermutation and selection during immune responses. This evolutionary process produces B cell clones, cells that descend from a common ancestor but differ by mutations. Phylogenetic trees inferred from BCR sequences can reconstruct the history of mutations within a clone. Until recently, BCR sequencing technologies separated heavy and light chains, but advancements in single cell sequencing now pair heavy and light chains from individual cells. However, it is unclear how these separate genes should be combined to infer B cell phylogenies. In this study, we investigated st

SUBMITTER: Jensen CG 

PROVIDER: S-EPMC10592837 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature