Reliable detection and quantification of plasmodesmal callose in Nicotiana benthamiana leaves during defense responses.

ABSTRACT: Callose, a beta-(1,3)-D-glucan polymer, is essential for regulating intercellular trafficking via plasmodesmata (PD). Pathogens manipulate PD-localized proteins to enable intercellular trafficking by removing callose at PD, or conversely by increasing callose accumulation at PD to limit intercellular trafficking during infection. Plant defense hormones like salicylic acid regulate PD-localized proteins to control PD and intercellular trafficking during innate immune defense responses such as systemic acquired resistance. Measuring callose deposition at PD in plants has therefore emerged as a popular parameter for assessing the intercellular trafficking activity during plant immunity. Despite the popularity of this metric there is no standard for how these measurements should be made. In th

SUBMITTER: Sankoh AF 

PROVIDER: S-EPMC10592870 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature