Lysosomal proteomics reveals mechanisms of neuronal apoE4-associated lysosomal dysfunction.

ABSTRACT: ApoE4 is the primary risk factor for Alzheimer Disease (AD). Early AD pathological events first affect the neuronal endolysosomal system, which in turn causes neuronal protein aggregation and cell death. Despite the crucial influence of lysosomes upon AD pathophysiology, and that apoE4 localizes to lysosomes, the influence of apoE4 on lysosomal function remains unexplored. We find that expression of apoE4 in neuronal cell lines results in lysosomal alkalinization and impaired lysosomal function. To identify driving factors for these defects, we performed quantitative lysosomal proteome profiling. This revealed that apoE4 expression results in differential regulation of numerous lysosomal proteins, correlating with apoE allele status and disease severity in AD brains. In particular, apoE4 e

SUBMITTER: Krogsaeter EK 

PROVIDER: S-EPMC10592882 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature