Synovial macrophage diversity and activation of M-CSF signaling in post-traumatic osteoarthritis.

ABSTRACT:

Objective

Synovium is home to immune and stromal cell types that orchestrate inflammation following a joint injury; in particular, macrophages are central protagonists in this process. We sought to define the cellular and temporal dynamics of the synovial immune niche in a mouse model of post-traumatic osteoarthritis (PTOA), and to identify stromal-immune crosstalk mechanisms that coordinate macrophage function and phenotype.

Design

We induced PTOA in mice using a non-invasive tibial compression model of anterior cruciate ligament rupture (ACLR). Single cell RNA-seq and flow cytometry were used to assess immune cell populations in healthy (Sham) and injured (7d and 28d post-ACLR) synovium. Characterization of synovial macrophage polarization states was performed, alongside c

SUBMITTER: Knights AJ 

PROVIDER: S-EPMC10592932 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature